Hệ thống phân tích định lượng PCR ( qPCR) REAL TIME PCR QuantStudio 6 and 7 Pro Real-Time PCR Systems dùng trong y học , thực phẫm hãng Applied Biosystem , hãng Thermo Fisher Scientific

Hệ thống phân tích định lượng PCR ( qPCR) REAL TIME PCR QuantStudio 6 and 7 Pro Real-Time PCR Systems dùng trong y học , thực phẫm hãng Applied Biosystem , hãng Thermo Fisher Scientific

Hệ thống phân tích định lượng PCR ( qPCR) REAL TIME PCR QuantStudio 6 and 7 Pro Real-Time PCR Systems dùng trong y học , thực phẫm hãng Applied Biosystem , hãng Thermo Fisher Scientific

QuantStudio qPCR Family of Instruments

Meet the new generation of qPCR instruments: as trusted and reliable as their forebears, but evolved with great new capabilities.

Picking a favorite family member is never easy, but the Applied Biosystems QuantStudio™ family of real-time and digital PCR systems has been designed so there’s an option suitable for every laboratory and every budget, whatever your needs.
Explore the entire family below.

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QuantStudio™
1 System

Entry-level with proven reliability

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QuantStudio®
3 System

Easy to use, access, and share your results

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QuantStudio®
6 Flex System

Flexibility designed to easily evolve with your research needs

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QuantStudio®
12K Flex System

High-throughput for maximum productivity with minimum effort

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QuantStudio®
5 System

Complete set of features to optimize your experience

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QuantStudio®
7 Flex System

Move from application to application with ease

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QuantStudio® 3D
Digital PCR System

Innovation designed to help you push the limits of PCR

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qPCR for who you are Applied Biosystems™ QuantStudio™ real-time PCR and digital PCR systems Every lab is unique. That’s why you deserve a qPCR platform that fits your needs. Perhaps you’re looking for simplicity on a budget, or reliable results from limited samples. Maybe your research requires high throughput for maximum productivity, or absolute answers to take your work to the next level. Whatever you need, there’s a QuantStudio qPCR system that’s just right for your research. Your research. Your way. QuantStudio real-time PCR and digital PCR systems Real-time PCR Real-time & digital PCR Digital PCR only QuantStudio™ 3 system QuantStudio™ 5 system QuantStudio™ 6 Flex system QuantStudio™ 7 Flex system QuantStudio™ 12K Flex system QuantStudio™ 3D system Price $ $$ $$ $$$ $$$$ $ Colors 4 colors 5, or 6 colors (21 filter combinations) 5 colors 6 colors (21 filter combinations) 6 colors (21 filter combinations) 2 colors (endpoint detection) Available formats 96-well (0.2 mL block) 96-well Fast (0.1 mL block) 96-well (0.2 mL block) 96-well Fast (0.1 mL block) 384-well 96-well 96-well Fast 384-well 96-well 96-well Fast 384-well TaqMan® Array card (384-well microfluidic card) 96-well 96-well Fast 384-well TaqMan Array card (384-well microfluidic card) OpenArray™ plates (3,072 through-holes) 20,000 partitions/chip Block change Fixed Fixed Block change from front in less than 1 minute, no tools required NA VeriFlex™ temperature control 3 zones 6 zones (96-well blocks only) NA Automation compatible No No No Yes Yes No Throughput Medium Medium Medium High Very high Low 21 CFR Part 11 enablement Security Security, auditing, e-signature package Optional security, auditing, e-signature packages available No Thermo Fisher Cloud Direct connection Connect to cloud through computer Touch screen Yes, interactive Yes, interactive Yes Yes Yes Yes Key applications • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • High resolution melt* • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • High resolution melt* • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Pharmacogenomics • Digital PCR • Quantification of molecular standards • Absolute quantification • Pathogen detection • Load determination • Copy number variation • Digital PCR Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 3 Your research. Your way. QuantStudio real-time PCR and digital PCR systems Real-time PCR Real-time & digital PCR Digital PCR only QuantStudio™ 3 system QuantStudio™ 5 system QuantStudio™ 6 Flex system QuantStudio™ 7 Flex system QuantStudio™ 12K Flex system QuantStudio™ 3D system Price $ $$ $$ $$$ $$$$ $ Colors 4 colors 5, or 6 colors (21 filter combinations) 5 colors 6 colors (21 filter combinations) 6 colors (21 filter combinations) 2 colors (endpoint detection) Available formats 96-well (0.2 mL block) 96-well Fast (0.1 mL block) 96-well (0.2 mL block) 96-well Fast (0.1 mL block) 384-well 96-well 96-well Fast 384-well 96-well 96-well Fast 384-well TaqMan® Array card (384-well microfluidic card) 96-well 96-well Fast 384-well TaqMan Array card (384-well microfluidic card) OpenArray™ plates (3,072 through-holes) 20,000 partitions/chip Block change Fixed Fixed Block change from front in less than 1 minute, no tools required NA VeriFlex™ temperature control 3 zones 6 zones (96-well blocks only) NA Automation compatible No No No Yes Yes No Throughput Medium Medium Medium High Very high Low 21 CFR Part 11 enablement Security Security, auditing, e-signature package Optional security, auditing, e-signature packages available No Thermo Fisher Cloud Direct connection Connect to cloud through computer Touch screen Yes, interactive Yes, interactive Yes Yes Yes Yes Key applications • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • High resolution melt* • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • High resolution melt* • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Pharmacogenomics • Digital PCR • Quantification of molecular standards • Absolute quantification • Pathogen detection • Load determination • Copy number variation • Digital PCR Key benefits Easy to use Intuitive software design enables easy experiment setup and an interactive system allows you to get results faster. The upgraded touch screen offers an enhanced experience, with increased touch sensitivity and natural interface navigation. Connect to the cloud Directly connect to Thermo Fisher Cloud to access and analyse your data anytime, from anywhere with an internet connection. Back up and share files to collaborate with colleagues worldwide. Precise temperature control Applied Biosystems™ VeriFlex™ technology on the QuantStudio 3 system provides three independent temperature zones for precise control over your qPCR optimisation. Ready to run Factory calibrated for optical and thermal accuracy, the instrument is delivered ready for quick installation and use. 4 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Application spotlight Identify more new variants quickly and accurately with high resolution melt High resolution melt (HRM) analysis is based on detecting small differences in PCR melting (dissociation) curves. It is enabled by improved dsDNA-binding dyes used in conjunction with real-time PCR instrumentation that has precise temperature ramp control and advanced data-capture capabilities. Achieve high-throughput HRM analysis using a QuantStudio qPCR system with Applied Biosystems™ MeltDoctor™ HRM Software built-in protocols, and calibrations. Advantages of HRM • Low reagent and sample consumption • Fast, simple workflow • Thermal optimisation not required QuantStudio 3 Real-Time PCR System The QuantStudio 3 Real-Time PCR System is a small, affordable solution connecting you to your data like never before. Designed to be the easiest to use and most interactive instrument from the QuantStudio instrument family, the QuantStudio 3 system offers the high-performance analysis expected from Applied Biosystems instruments. I need ultimate simplicity Intuitive and easy use for all levels of experience. 5 The QuantStudio 3 system is simple, affordable and designed to get users with all levels of experience up and running quickly. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Key benefits Multiplex with ease Six excitation filters and six emission filters offer 21 different color combinations, allowing a broad range of detection chemistries and maximum multiplexing.* Connect to the cloud Directly connect to Thermo Fisher Cloud to access and analyse your data anytime, from anywhere, with an internet connection. Back up and share files to collaborate with colleagues worldwide. Precise temperature control VeriFlex™ technology on the QuantStudio 5 system provides six independent temperature zones for precise control over your PCR optimisation.* Confidence in your records Built-in security, auditing, and electronic signature (SAE) package comes standard on the QuantStudio 5 system to assist you in 21 CFR Part 11 compliance. Protect your work Individual user accounts and the ability to lock a protocol template gives you peace of mind that only you control your runs. Better than gradient with Applied Biosystems™ VeriFlex™ Blocks VeriFlex technology provides independent temperature zones that offer enhanced functionality and precise control over your qPCR runs. The QuantStudio 3 system has three programmable zones and the QuantStudio 5 system has six programmable zones for the 96-well standard and 96-well Fast blocks. These independent zones are ideal for qPCR optimisation or the ability to run multiple experiments in the same run. Unlike standard gradients which give a sigmoidal temperature curve across the columns, VeriFlex Blocks help deliver accurate temperatures across each and every zone. *384-well format includes 5 colors, no VeriFlex Block temperature control 6 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Application spotlight QuantStudio 5 Real-Time PCR System The QuantStudio 5 Real-Time PCR System is a high-performance benchtop instrument giving you greater control of your experiment data. Interact with the latest advancements in touch screen usability, access your data more easily than ever before and securely share your results with collaborators around the world. I need more control Take charge of how you create and manage your results. The VeriFlex Block provides independent temperature zones. 7 The QuantStudio 5 system combines ease of use and system connectivity with powerful options to enable maximum control. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 8 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Key benefits Flexibility that minimises large up-front capital investment With one instrument, you can interchange between 96-well, 96-well Fast, and 384-well formats. Skip the learning curve The intuitive software, easy touch-screen setup, and effortless block change are designed to let you get started right away. Performance you can trust Enables detection as small as 1.5-fold changes in singleplex reactions and with 10 logs dynamic range. Upgrade capabilities when you need it The QuantStudio 6 Flex system can be seamlessly upgraded to a QuantStudio 7 Flex by a field service engineer to give you additional automation, throughput, and multiplexing capabilities. Application spotlight QuantStudio 6 Flex Real-Time PCR System The QuantStudio 6 Flex Real-Time PCR System is ideal for laboratories with multiple applications or changing throughput needs. With an upgradable path to a QuantStudio 7 Flex system to accommodate automation or TaqMan Array cards, the QuantStudio 6 Flex system is the ideal qPCR platform if your needs change in the future. I need room to grow Flexibility for today, tomorrow and beyond. Analyse protein melt profiles and protein thermal stability Applied Biosystems™ Protein Thermal Shift™ software and reagents enable a real-time PCR system solution for differential scanning fluorometry. Run a real-time protein melt experiment to screen for ligand-protein binding, optimise buffer conditions, or identify protein stability changes. The Protein Thermal Shift solution offers a highthroughput and inexpensive alternative to traditional methods. Advantages of the Protein Thermal Shift solution • Results in as little as five minutes • User-friendly software for differential scanning fluorometry • Run thousands of reactions per day at pennies per reaction Fluorescence 30.0 32.5 35.0 37.5 40.0 42.5 45.0 47.5 50.0 52.5 55.0 0 40,000 20,000 60,000 80,000 100,000 Protein Protein + Ligand d(Fluorescence)/dT 30.0 32.5 35.0 37.5 40.0 42.5 45.0 47.5 50.0 52.5 55.0 0 -5,000 5,000 10,000 15,000 Temperature (oC) ~7oC Tm Difference Fluorescence melt curve plot and derivative curve plot for a protein before (red) and after (blue) a ligand is added. 9 The QuantStudio 6 Flex system gives you flexibility at an affordable price. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 9 10 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Key benefits Accomplish more, faster Run hundreds of real-time PCR reactions using TaqMan Array microfluidic cards integrated with an automation robot. The QuantStudio 7 Flex system can help maximise your throughput capabilities for automated environments. High application versatility Offers optimised protocols, reagents, and intuitive software for the broadest range of applications, including: Get results you can trust Enabling improved well-to-well and instrument-toinstrument data accuracy, the Applied Biosystems™ OptiFlex™ System features six decoupled excitation and emission filter channels, with 21 filter combinations for maximum multiplexing and chemistry flexibility. Application spotlight I need more versatility Moving from application to application is now easier than ever with additional dyes, formats and automation potential. QuantStudio 7 Flex Real-Time PCR System The QuantStudio 7 Flex Real-Time PCR System delivers reliability, sensitivity, and accuracy with the versatility of four interchangeable blocks. The QuantStudio 7 Flex system is optimised to enable the broadest range of quantitative PCR applications. • Gene expression • Long non-coding RNA analysis • Pri-miRNA analysis • Mutation detection • High resolution melt • SNP genotyping • MicroRNA profiling • Protein thermal shift Identification of novel pathways contributing to immune response The Applied Biosystems™ TaqMan® Human Immune Array Card is a costeffective, easy-to-use microfluidic card for quantitative gene expression analysis of targets known to have implications in immune response. The gene targets on the TaqMan Human Immune Array Card include cytokines, chemokines, growth factors, immune regulators, apoptosis markers, ischemia markers, tissue-specific markers, and others, including classic endogenous markers. The array is cost-effective, convenient, and easy to use without needing expensive robotics. It enables reproducible and consistent results across samples, studies, and labs—providing the same data quality from card to card and lot to lot—even with different operators. Hundreds of customisable and predefined TaqMan Array microfluidic cards are available, containing preloaded Applied Biosystems™ TaqMan® Gene Expression or MicroRNA Assays in an easy-to-use format. Life Technologies | QuantStudio 11 ® Real-Time PCR and Digital PCR Systems Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 11 Help advance your research further with the application versatility of the QuantStudio 7 Flex system. 12 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 1. Load samples onto OpenArray plate 2. Cycle & image OpenArray plates 3. Analyse results Up to 1,100 samples per 8 hr day AlleleTyper™ Software for star allele reports Minimal hands-on time QuantStudio 12K Flex Real-Time PCR System With this one instrument, your lab can be ready for many types of experiments and users, from low- to high-throughput sample processing and virtually any PCR application, such as: • Drug discovery • Pharmaceutical target confirmation • MicroRNA profiling • Agriculture molecular testing Key benefits Miniaturisation at lower cost Applied Biosystems™ OpenArray™ technology is a broadly applicable nanoliter fluidics platform for low-volume, solution-phase reactions, and enables lower reagent and assay costs, and rapid parallel processing. Unparalleled throughput The QuantStudio 12K Flex system can simultaneously run up to four 3,072-reaction OpenArray plates in about 4 hours. Produce up to 110,000 data points in an 8-hour day with the addition of a single Applied Biosystems™ ProFlex™ PCR System. Outstanding flexibility Seamlessly switch between five available thermal cycling blocks: OpenArray plate, Applied Biosystems™ TaqMan® Array card, 384-well, and standard or Fast 96-well blocks. Choose and load the thermal-cycling block typically in less than 1 minute and with no required tools. Increased data integrity and quality control— with results you can trust The integrated sample tracking and security, auditing, and electronic signature (SAE) module assist you in 21 CFR Part 11 compliance. Pharmacogenomics Pharmacogenomics is the study of drug efficacy based on individuals’ unique genomic composition. The QuantStudio 12K Flex system and OpenArray technology provide a simple, cost-effective, and fast screening workflow for the analysis of mutations and copy number variants for drug metabolism enzyme (DME) genes. I need maximum productivity Harness the power of high-throughput technology for maximum productivity with minimum effort. Application spotlight 13 13 With the QuantStudio 12K Flex system and OpenArray technology, a single user can generate from 1 to over 12,000 data points in a single run with minimal hands-on time. Applied Biosystems | QuantStudioreal-time PCR and digital PCR systems 14 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems QuantStudio 3D Digital PCR System The QuantStudio 3D Digital PCR System leverages high-density nanofluidic chip technology for investigations that involve detecting rare events, analyzing small differences between two targets, or counting the exact number of targets in a sample. Due to the higher precision, sensitivity, and absolute nature of digital PCR, it is ideal for the following applications: • Qualification of molecular standards used in traditional real-time PCR experiments • Pathogen detection and load determination • Rare-target detection such as somatic mutation detection in oncology research • GMO detection and contamination assessment • Generation of references and standards • Copy number variation I need absolute answers Take your research to the next level with technology that is as innovative as your research. Determining low copy number in equivocal tissue Many cancers are regulated by mutations in a specific gene or group of genes, or copy number changes. These aberrations can be associated with aggressiveness of the disease or prognosis. Digital PCR provides a fast and easy workflow to precisely identify low copy numbers that have small differences. Compared to immunohistochemistry by fluorescence in situ hybridisation, digital PCR is less impacted by tissue heterogeneity and provides clearer results. Sample partitioned into many reactions Absolute quantification 100% Positive reaction Negative reaction Affordable Less than half the price of competing platforms, making new digital PCR technology within reach for most labs. Absolute quantification 20,000 reaction wells yield data in copies/μL enabling high precision and sensitivity without the need for a standard curve. Sealed system Helps limit contaminants with a sealed chip and no exposed sample transfer steps. Easily fits in populated spaces Small footprint (7 x 5 x 9 in.) will fit most benchtops and can be moved when needed. Compatible Use your existing Applied Biosystems™ TaqMan® Real-Time PCR Assays for a digital result. Key benefits Simple workflow A streamlined workflow with minimal sample handling enables turnkey processing. Just load and go. Application spotlight 15 15 The QuantStudio 3D Digital PCR System enables sensitive and precise absolute target quantification without the use of a reference or standard curve. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 16 Analysis software We offer Applied Biosystems primary and secondary analysis software for real-time PCR and digital PCR applications. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Software and description QuantStudio 3 QuantStudio 5 QuantStudio 6 Flex QuantStudio 7 Flex QuantStudio 12 Flex QuantStudio 3D Web browser–based primary analysis software • • • • • • Desktop primary analysis software • • • • • Sample Tracker Software facilitates sample loading onto OpenArray plate • HRM Software module for 96-well and 384-well blocks • • • • • Security, Auditing and eSignature (SAE) software (to support 21 CFR part 11 workflow) • • • • ExpressionSuite™ Software for enhanced gene expression analysis • • • Genotyper™ Software for SNP genotyping data analysis • • • DigitalSuite™ Software to switch from real-time to digital PCR data analysis • QuantStudio™ 3D AnalysisSuite™ Software for data QC and multi-chip analysis • Protein Thermal Shift (PTS) Software • • • 17 Applied Biosystems™ Analysis Modules Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Applied Biosystems™ Analysis Modules are innovative cloud-based data analysis applications that bring together multiple data sets in one convenient place for enhanced analysis and insights. Experience unprecedented ease of use by working from anywhere, with any operating system, on any computer connected to the internet. Get access to the latest secondary data analysis technology with updated, improved, and always current software modules for gene expression, genotyping, and absolute quantitation. Combine hundreds of qPCR experiments into a project and analyse the data within minutes. Simplify your workflow and spend less time on data analysis, so you can invest your valuable time publishing your results and advancing your research. • Data accessible with any device • All experiments integrated into one project • MIQE guideline support • Powered by Amazon Web Services™ Meet the needs of your laboratory today and tomorrow. For more information, go to thermofisher.com/abmodules 18 Real-time PCR application areas Real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimised master mixes, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Discover solutions for your qPCR-based research. Infectious disease research See our growing catalog of sensitive, specific real-time PCR probe and primer sets for human viruses and other infectious disease research. Food pathogen detection Detect multiple bacteria in the same run, including Salmonella, Campylobacter, E. coli O157:H7, and Listeria monocytogenes. Waterborne pathogen detection Designed to detect and monitor waterborne pathogens in recreational and drinking water supplies. Pharmaceutical analytics Designed to detect mycoplasmas, viruses, and residual host cell contamination for pharmaceutical, cosmetics, and personal care product manufacturing. Stem cell research Solutions for analyzing stem cells, determining stemness, and studying gene regulation and translation in stem cells. Pharmacogenomics Predesigned TaqMan Assays for more than 175 ADME and CYP targets, including >95% of ADME core markers and a warfarin metabolism panel. Oncology and genetic disease research Enabling robust, reliable detection and quantitation of markers for cancer and genetic diseases. Plant sciences and agricultural biotechnology Instruments, reagents, and kits designed for plant researchers that enable remarkable agricultural discoveries—such as improved crops that feed more people to sustainable biofuels. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 19 TaqMan chemistry and SYBR Green chemistry for real-time PCR TaqMan Assay reagents SYBR Green reagents Specificity High Low Sensitivity—low copy number High Variable* Reproducibility High Variable* Multiplexing Yes No Predesigned assays Yes No Custom assays Yes No User design and optimisation No Yes Cost High Low* Gene expression quantitation High Low DNA quantitation Yes Yes (pathogen detection) ChIP Yes Yes SNP genotyping Yes No MicroRNA Yes No Copy number Yes No Somatic mutation detection Yes No Pathway analysis Yes No Digital PCR Yes No *Depends on template quality, and primer design and optimisation TaqMan Assay—based detection SYBR Green—based detection Chemistry overview Uses a fluorogenic probe to enable detection of a specific PCR product as it accumulates during PCR cycles Uses SYBR™ Green I, or similar dye that binds to double-stranded DNA to detect PCR product as it accumulates during PCR We offer two types of chemistries to detect PCR products using real-time PCR instruments: • TaqMan Assay chemistry (also known as fluorogenic 5’ nuclease chemistry) • SYBR Green I dye chemistry Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 20 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Ordering information QuantStudio 6 Flex Real-Time PCR System configurations Cat. No. QuantStudio 6 Flex 96-well instrument, laptop configuration 4485689 QuantStudio 6 Flex 96-well Fast instrument, laptop configuration 4485699 QuantStudio 6 Flex 384-well instrument, laptop configuration 4485691 QuantStudio 6 Flex 96-well instrument, desktop configuration 4485692 QuantStudio 6 Flex 96-well Fast instrument, desktop configuration 4485697 QuantStudio 6 Flex 384-well instrument, desktop configuration 4485694 QuantStudio 6/7 Flex 96-well block upgrade kit 4453543 QuantStudio 6/7 Flex 96-well Fast block upgrade kit 4453544 QuantStudio 6/7 Flex 384-well block upgrade kit 4453545 QuantStudio 3 Real-Time PCR System configurations Cat. No. QuantStudio 3 96-well (0.2 mL block) instrument* A28137 QuantStudio 3 96-well Fast (0.1 mL block) instrument* A28136 QuantStudio 5 Real-Time PCR System configurations Cat. No. QuantStudio 5 96-well (0.2 mL block) instrument* A28139 QuantStudio 5 96-well Fast (0.1 mL block) instrument* A28138 QuantStudio 5 384-well instrument* A28140 QuantStudio 7 Flex Real-Time PCR System configurations Cat. No. QuantStudio 7 Flex 96-well instrument, laptop configuration 4485688 QuantStudio 7 Flex 96-well Fast instrument, laptop configuration 4485698 QuantStudio 7 Flex 384-well instrument, laptop configuration 4485695 QuantStudio 7 Flex TaqMan Array Card instrument, laptop configuration 4485700 QuantStudio 7 Flex 96-well instrument, desktop configuration 4485690 QuantStudio 7 Flex 96-well Fast instrument, desktop configuration 4485693 QuantStudio 7 Flex 384-well instrument, desktop configuration 4485701 QuantStudio 7 Flex TaqMan Array Card instrument, desktop configuration 4485696 QuantStudio 6/7 Flex 96-well block upgrade kit 4453543 QuantStudio 6/7 Flex 96-well Fast block upgrade kit 4453544 QuantStudio 6/7 Flex 384-well block upgrade kit 4453545 QuantStudio 7 Flex TaqMan Array Card block upgrade kit 4453546 * Does not include computer. Additional Cat. Nos. are available that include laptop or desktop computer. * Does not include computer. Additional Cat. Nos. are available that include laptop or desktop computer. 21 QuantStudio 12K Flex System configurations Cat. No. QuantStudio 12K Flex OpenArray block includes AccuFill™ System, laptop configuration 4471086 QuantStudio 12K Flex OpenArray block without AccuFill System, laptop configuration 4472379 QuantStudio 12K Flex 96-well instrument, laptop configuration 4471050 QuantStudio 12K Flex 96-well Fast instrument, laptop configuration 4471080 QuantStudio 12K Flex 384-well instrument, laptop configuration 4471081 QuantStudio 12K Flex, TaqMan Array Card instrument, laptop configuration 4471085 QuantStudio 12K Flex OpenArray block includes AccuFillSystem, desktop configuration 4471090 QuantStudio 12K Flex OpenArray block without AccuFill System, desktop configuration 4472380 QuantStudio 12K Flex 96-well instrument, desktop configuration 4471087 QuantStudio 12K Flex 96-well Fast instrument, desktop configuration 4471088 QuantStudio 12K Flex 384-well instrument, desktop configuration 4471134 QuantStudio 12K Flex TaqMan Array Card instrument, desktop configuration 4471089 QuantStudio 12K Flex 96-well block upgrade kit 4453543 QuantStudio 12K Flex 96-well Fast block upgrade kit 4453544 QuantStudio 12K Flex 384-well block upgrade kit 4453545 QuantStudio 12K Flex TaqMan Array Card block upgrade kit 4453546 QuantStudio 12K Flex OpenArray block upgrade kit with AccuFill System 4471067 QuantStudio 12K Flex AccuFill System 4471021 ProFlex™ Dual Flat PCR System 4484078 QuantStudio 3D Digital PCR System Cat. No. QuantStudio 3D Digital PCR System Package—includes: A25581 QuantStudio 3D Digital PCR instrument 4481097 QuantStudio 3D Digital PCR Chip Loader 4482592 QuantStudio 3D Digital PCR Chip Adapter Kit for Flat Block Thermal Cycler 4485513 QuantStudio 3D Digital PCR 20K Chip Pack (includes consumables) 4485507 ProFlex Dual Flat PCR System 4484078 QuantStudio 3D Digital PCR Master Mix (1.5 mL) 4482710 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 22 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Support at every step Online Instrument Management Sign in to your thermofisher.com account to access the award-winning* free online Instrument Management** tool that enables faster responses to requests for service or service quotes, plus instant connection to key instrument and service information. Comprehensive instrument warranty Our factory-trained and certified field service engineers (FSEs) are focused on delivering the highest quality workmanship. During the warranty period all repairs, including engineer time and travel, are covered. Flexible service plans Choose from a variety of service options that balance your budget, productivity, uptime and regulatory requirements. Plans start with the most basic repair models and scale to premium offerings including advanced support and compliance services. On-site service plans are optimal for labs that have time-sensitive work and need to get their instrument back online quickly. These plans include guaranteed response times in most regions, scheduled planned maintenance and automatic software updates. The AB Repair Center plan is the cost-effective choice for customers who can allow their instrument to be sent away for repair—this plan provides a loaner instrument so that customers can maintain productivity while their instrument is being repaired. Compliance and validation services Our compliance and validation services are designed to help you balance business and regulatory requirements. From risk assessment and hardware/software qualification to full system validation, we partner with you to help mitigate regulatory risks and get your processes up and running. Training courses Our application and instrument training programs are led by scientists who aim to enhance your workday through experimental design best practices, workflow training and instrument troubleshooting. Hands-on classes are available at our Thermo Fisher Scientific training centers or in your lab. Technical support If you have questions about product selection or use, assay or experiment design, data analysis, or troubleshooting, contact our team of technical support scientists or access our online product and application support tools

Hệ thống phân tích định lượng PCR ( qPCR) REAL TIME PCR QuantStudio 6 and 7 Pro Real-Time PCR Systems dùng trong y học , thực phẫm hãng Applied Biosystem , hãng Thermo Fisher Scientific

qPCR for who you are Applied Biosystems™ QuantStudio™ real-time PCR and digital PCR systems Every lab is unique. That’s why you deserve a qPCR platform that fits your needs. Perhaps you’re looking for simplicity on a budget, or reliable results from limited samples. Maybe your research requires high throughput for maximum productivity, or absolute answers to take your work to the next level. Whatever you need, there’s a QuantStudio qPCR system that’s just right for your research. Your research. Your way. QuantStudio real-time PCR and digital PCR systems Real-time PCR Real-time & digital PCR Digital PCR only QuantStudio™ 3 system QuantStudio™ 5 system QuantStudio™ 6 Flex system QuantStudio™ 7 Flex system QuantStudio™ 12K Flex system QuantStudio™ 3D system Price $ $$ $$ $$$ $$$$ $ Colors 4 colors 5, or 6 colors (21 filter combinations) 5 colors 6 colors (21 filter combinations) 6 colors (21 filter combinations) 2 colors (endpoint detection) Available formats 96-well (0.2 mL block) 96-well Fast (0.1 mL block) 96-well (0.2 mL block) 96-well Fast (0.1 mL block) 384-well 96-well 96-well Fast 384-well 96-well 96-well Fast 384-well TaqMan® Array card (384-well microfluidic card) 96-well 96-well Fast 384-well TaqMan Array card (384-well microfluidic card) OpenArray™ plates (3,072 through-holes) 20,000 partitions/chip Block change Fixed Fixed Block change from front in less than 1 minute, no tools required NA VeriFlex™ temperature control 3 zones 6 zones (96-well blocks only) NA Automation compatible No No No Yes Yes No Throughput Medium Medium Medium High Very high Low 21 CFR Part 11 enablement Security Security, auditing, e-signature package Optional security, auditing, e-signature packages available No Thermo Fisher Cloud Direct connection Connect to cloud through computer Touch screen Yes, interactive Yes, interactive Yes Yes Yes Yes Key applications • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • High resolution melt* • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • High resolution melt* • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Pharmacogenomics • Digital PCR • Quantification of molecular standards • Absolute quantification • Pathogen detection • Load determination • Copy number variation • Digital PCR Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 3 Your research. Your way. QuantStudio real-time PCR and digital PCR systems Real-time PCR Real-time & digital PCR Digital PCR only QuantStudio™ 3 system QuantStudio™ 5 system QuantStudio™ 6 Flex system QuantStudio™ 7 Flex system QuantStudio™ 12K Flex system QuantStudio™ 3D system Price $ $$ $$ $$$ $$$$ $ Colors 4 colors 5, or 6 colors (21 filter combinations) 5 colors 6 colors (21 filter combinations) 6 colors (21 filter combinations) 2 colors (endpoint detection) Available formats 96-well (0.2 mL block) 96-well Fast (0.1 mL block) 96-well (0.2 mL block) 96-well Fast (0.1 mL block) 384-well 96-well 96-well Fast 384-well 96-well 96-well Fast 384-well TaqMan® Array card (384-well microfluidic card) 96-well 96-well Fast 384-well TaqMan Array card (384-well microfluidic card) OpenArray™ plates (3,072 through-holes) 20,000 partitions/chip Block change Fixed Fixed Block change from front in less than 1 minute, no tools required NA VeriFlex™ temperature control 3 zones 6 zones (96-well blocks only) NA Automation compatible No No No Yes Yes No Throughput Medium Medium Medium High Very high Low 21 CFR Part 11 enablement Security Security, auditing, e-signature package Optional security, auditing, e-signature packages available No Thermo Fisher Cloud Direct connection Connect to cloud through computer Touch screen Yes, interactive Yes, interactive Yes Yes Yes Yes Key applications • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • High resolution melt* • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • High resolution melt* • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Gene expression • miRNA profiling • SNP genotyping • Copy number variation • Protein thermal shift • High resolution melt • Pathogen detection • Pharmacogenomics • Digital PCR • Quantification of molecular standards • Absolute quantification • Pathogen detection • Load determination • Copy number variation • Digital PCR Key benefits Easy to use Intuitive software design enables easy experiment setup and an interactive system allows you to get results faster. The upgraded touch screen offers an enhanced experience, with increased touch sensitivity and natural interface navigation. Connect to the cloud Directly connect to Thermo Fisher Cloud to access and analyse your data anytime, from anywhere with an internet connection. Back up and share files to collaborate with colleagues worldwide. Precise temperature control Applied Biosystems™ VeriFlex™ technology on the QuantStudio 3 system provides three independent temperature zones for precise control over your qPCR optimisation. Ready to run Factory calibrated for optical and thermal accuracy, the instrument is delivered ready for quick installation and use. 4 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Application spotlight Identify more new variants quickly and accurately with high resolution melt High resolution melt (HRM) analysis is based on detecting small differences in PCR melting (dissociation) curves. It is enabled by improved dsDNA-binding dyes used in conjunction with real-time PCR instrumentation that has precise temperature ramp control and advanced data-capture capabilities. Achieve high-throughput HRM analysis using a QuantStudio qPCR system with Applied Biosystems™ MeltDoctor™ HRM Software built-in protocols, and calibrations. Advantages of HRM • Low reagent and sample consumption • Fast, simple workflow • Thermal optimisation not required QuantStudio 3 Real-Time PCR System The QuantStudio 3 Real-Time PCR System is a small, affordable solution connecting you to your data like never before. Designed to be the easiest to use and most interactive instrument from the QuantStudio instrument family, the QuantStudio 3 system offers the high-performance analysis expected from Applied Biosystems instruments. I need ultimate simplicity Intuitive and easy use for all levels of experience. 5 The QuantStudio 3 system is simple, affordable and designed to get users with all levels of experience up and running quickly. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Key benefits Multiplex with ease Six excitation filters and six emission filters offer 21 different color combinations, allowing a broad range of detection chemistries and maximum multiplexing.* Connect to the cloud Directly connect to Thermo Fisher Cloud to access and analyse your data anytime, from anywhere, with an internet connection. Back up and share files to collaborate with colleagues worldwide. Precise temperature control VeriFlex™ technology on the QuantStudio 5 system provides six independent temperature zones for precise control over your PCR optimisation.* Confidence in your records Built-in security, auditing, and electronic signature (SAE) package comes standard on the QuantStudio 5 system to assist you in 21 CFR Part 11 compliance. Protect your work Individual user accounts and the ability to lock a protocol template gives you peace of mind that only you control your runs. Better than gradient with Applied Biosystems™ VeriFlex™ Blocks VeriFlex technology provides independent temperature zones that offer enhanced functionality and precise control over your qPCR runs. The QuantStudio 3 system has three programmable zones and the QuantStudio 5 system has six programmable zones for the 96-well standard and 96-well Fast blocks. These independent zones are ideal for qPCR optimisation or the ability to run multiple experiments in the same run. Unlike standard gradients which give a sigmoidal temperature curve across the columns, VeriFlex Blocks help deliver accurate temperatures across each and every zone. *384-well format includes 5 colors, no VeriFlex Block temperature control 6 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Application spotlight QuantStudio 5 Real-Time PCR System The QuantStudio 5 Real-Time PCR System is a high-performance benchtop instrument giving you greater control of your experiment data. Interact with the latest advancements in touch screen usability, access your data more easily than ever before and securely share your results with collaborators around the world. I need more control Take charge of how you create and manage your results. The VeriFlex Block provides independent temperature zones. 7 The QuantStudio 5 system combines ease of use and system connectivity with powerful options to enable maximum control. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 8 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Key benefits Flexibility that minimises large up-front capital investment With one instrument, you can interchange between 96-well, 96-well Fast, and 384-well formats. Skip the learning curve The intuitive software, easy touch-screen setup, and effortless block change are designed to let you get started right away. Performance you can trust Enables detection as small as 1.5-fold changes in singleplex reactions and with 10 logs dynamic range. Upgrade capabilities when you need it The QuantStudio 6 Flex system can be seamlessly upgraded to a QuantStudio 7 Flex by a field service engineer to give you additional automation, throughput, and multiplexing capabilities. Application spotlight QuantStudio 6 Flex Real-Time PCR System The QuantStudio 6 Flex Real-Time PCR System is ideal for laboratories with multiple applications or changing throughput needs. With an upgradable path to a QuantStudio 7 Flex system to accommodate automation or TaqMan Array cards, the QuantStudio 6 Flex system is the ideal qPCR platform if your needs change in the future. I need room to grow Flexibility for today, tomorrow and beyond. Analyse protein melt profiles and protein thermal stability Applied Biosystems™ Protein Thermal Shift™ software and reagents enable a real-time PCR system solution for differential scanning fluorometry. Run a real-time protein melt experiment to screen for ligand-protein binding, optimise buffer conditions, or identify protein stability changes. The Protein Thermal Shift solution offers a highthroughput and inexpensive alternative to traditional methods. Advantages of the Protein Thermal Shift solution • Results in as little as five minutes • User-friendly software for differential scanning fluorometry • Run thousands of reactions per day at pennies per reaction Fluorescence 30.0 32.5 35.0 37.5 40.0 42.5 45.0 47.5 50.0 52.5 55.0 0 40,000 20,000 60,000 80,000 100,000 Protein Protein + Ligand d(Fluorescence)/dT 30.0 32.5 35.0 37.5 40.0 42.5 45.0 47.5 50.0 52.5 55.0 0 -5,000 5,000 10,000 15,000 Temperature (oC) ~7oC Tm Difference Fluorescence melt curve plot and derivative curve plot for a protein before (red) and after (blue) a ligand is added. 9 The QuantStudio 6 Flex system gives you flexibility at an affordable price. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 9 10 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Key benefits Accomplish more, faster Run hundreds of real-time PCR reactions using TaqMan Array microfluidic cards integrated with an automation robot. The QuantStudio 7 Flex system can help maximise your throughput capabilities for automated environments. High application versatility Offers optimised protocols, reagents, and intuitive software for the broadest range of applications, including: Get results you can trust Enabling improved well-to-well and instrument-toinstrument data accuracy, the Applied Biosystems™ OptiFlex™ System features six decoupled excitation and emission filter channels, with 21 filter combinations for maximum multiplexing and chemistry flexibility. Application spotlight I need more versatility Moving from application to application is now easier than ever with additional dyes, formats and automation potential. QuantStudio 7 Flex Real-Time PCR System The QuantStudio 7 Flex Real-Time PCR System delivers reliability, sensitivity, and accuracy with the versatility of four interchangeable blocks. The QuantStudio 7 Flex system is optimised to enable the broadest range of quantitative PCR applications. • Gene expression • Long non-coding RNA analysis • Pri-miRNA analysis • Mutation detection • High resolution melt • SNP genotyping • MicroRNA profiling • Protein thermal shift Identification of novel pathways contributing to immune response The Applied Biosystems™ TaqMan® Human Immune Array Card is a costeffective, easy-to-use microfluidic card for quantitative gene expression analysis of targets known to have implications in immune response. The gene targets on the TaqMan Human Immune Array Card include cytokines, chemokines, growth factors, immune regulators, apoptosis markers, ischemia markers, tissue-specific markers, and others, including classic endogenous markers. The array is cost-effective, convenient, and easy to use without needing expensive robotics. It enables reproducible and consistent results across samples, studies, and labs—providing the same data quality from card to card and lot to lot—even with different operators. Hundreds of customisable and predefined TaqMan Array microfluidic cards are available, containing preloaded Applied Biosystems™ TaqMan® Gene Expression or MicroRNA Assays in an easy-to-use format. Life Technologies | QuantStudio 11 ® Real-Time PCR and Digital PCR Systems Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 11 Help advance your research further with the application versatility of the QuantStudio 7 Flex system. 12 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 1. Load samples onto OpenArray plate 2. Cycle & image OpenArray plates 3. Analyse results Up to 1,100 samples per 8 hr day AlleleTyper™ Software for star allele reports Minimal hands-on time QuantStudio 12K Flex Real-Time PCR System With this one instrument, your lab can be ready for many types of experiments and users, from low- to high-throughput sample processing and virtually any PCR application, such as: • Drug discovery • Pharmaceutical target confirmation • MicroRNA profiling • Agriculture molecular testing Key benefits Miniaturisation at lower cost Applied Biosystems™ OpenArray™ technology is a broadly applicable nanoliter fluidics platform for low-volume, solution-phase reactions, and enables lower reagent and assay costs, and rapid parallel processing. Unparalleled throughput The QuantStudio 12K Flex system can simultaneously run up to four 3,072-reaction OpenArray plates in about 4 hours. Produce up to 110,000 data points in an 8-hour day with the addition of a single Applied Biosystems™ ProFlex™ PCR System. Outstanding flexibility Seamlessly switch between five available thermal cycling blocks: OpenArray plate, Applied Biosystems™ TaqMan® Array card, 384-well, and standard or Fast 96-well blocks. Choose and load the thermal-cycling block typically in less than 1 minute and with no required tools. Increased data integrity and quality control— with results you can trust The integrated sample tracking and security, auditing, and electronic signature (SAE) module assist you in 21 CFR Part 11 compliance. Pharmacogenomics Pharmacogenomics is the study of drug efficacy based on individuals’ unique genomic composition. The QuantStudio 12K Flex system and OpenArray technology provide a simple, cost-effective, and fast screening workflow for the analysis of mutations and copy number variants for drug metabolism enzyme (DME) genes. I need maximum productivity Harness the power of high-throughput technology for maximum productivity with minimum effort. Application spotlight 13 13 With the QuantStudio 12K Flex system and OpenArray technology, a single user can generate from 1 to over 12,000 data points in a single run with minimal hands-on time. Applied Biosystems | QuantStudioreal-time PCR and digital PCR systems 14 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems QuantStudio 3D Digital PCR System The QuantStudio 3D Digital PCR System leverages high-density nanofluidic chip technology for investigations that involve detecting rare events, analyzing small differences between two targets, or counting the exact number of targets in a sample. Due to the higher precision, sensitivity, and absolute nature of digital PCR, it is ideal for the following applications: • Qualification of molecular standards used in traditional real-time PCR experiments • Pathogen detection and load determination • Rare-target detection such as somatic mutation detection in oncology research • GMO detection and contamination assessment • Generation of references and standards • Copy number variation I need absolute answers Take your research to the next level with technology that is as innovative as your research. Determining low copy number in equivocal tissue Many cancers are regulated by mutations in a specific gene or group of genes, or copy number changes. These aberrations can be associated with aggressiveness of the disease or prognosis. Digital PCR provides a fast and easy workflow to precisely identify low copy numbers that have small differences. Compared to immunohistochemistry by fluorescence in situ hybridisation, digital PCR is less impacted by tissue heterogeneity and provides clearer results. Sample partitioned into many reactions Absolute quantification 100% Positive reaction Negative reaction Affordable Less than half the price of competing platforms, making new digital PCR technology within reach for most labs. Absolute quantification 20,000 reaction wells yield data in copies/μL enabling high precision and sensitivity without the need for a standard curve. Sealed system Helps limit contaminants with a sealed chip and no exposed sample transfer steps. Easily fits in populated spaces Small footprint (7 x 5 x 9 in.) will fit most benchtops and can be moved when needed. Compatible Use your existing Applied Biosystems™ TaqMan® Real-Time PCR Assays for a digital result. Key benefits Simple workflow A streamlined workflow with minimal sample handling enables turnkey processing. Just load and go. Application spotlight 15 15 The QuantStudio 3D Digital PCR System enables sensitive and precise absolute target quantification without the use of a reference or standard curve. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 16 Analysis software We offer Applied Biosystems primary and secondary analysis software for real-time PCR and digital PCR applications. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Software and description QuantStudio 3 QuantStudio 5 QuantStudio 6 Flex QuantStudio 7 Flex QuantStudio 12 Flex QuantStudio 3D Web browser–based primary analysis software • • • • • • Desktop primary analysis software • • • • • Sample Tracker Software facilitates sample loading onto OpenArray plate • HRM Software module for 96-well and 384-well blocks • • • • • Security, Auditing and eSignature (SAE) software (to support 21 CFR part 11 workflow) • • • • ExpressionSuite™ Software for enhanced gene expression analysis • • • Genotyper™ Software for SNP genotyping data analysis • • • DigitalSuite™ Software to switch from real-time to digital PCR data analysis • QuantStudio™ 3D AnalysisSuite™ Software for data QC and multi-chip analysis • Protein Thermal Shift (PTS) Software • • • 17 Applied Biosystems™ Analysis Modules Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Applied Biosystems™ Analysis Modules are innovative cloud-based data analysis applications that bring together multiple data sets in one convenient place for enhanced analysis and insights. Experience unprecedented ease of use by working from anywhere, with any operating system, on any computer connected to the internet. Get access to the latest secondary data analysis technology with updated, improved, and always current software modules for gene expression, genotyping, and absolute quantitation. Combine hundreds of qPCR experiments into a project and analyse the data within minutes. Simplify your workflow and spend less time on data analysis, so you can invest your valuable time publishing your results and advancing your research. • Data accessible with any device • All experiments integrated into one project • MIQE guideline support • Powered by Amazon Web Services™ Meet the needs of your laboratory today and tomorrow. For more information, go to thermofisher.com/abmodules 18 Real-time PCR application areas Real-time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimised master mixes, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Discover solutions for your qPCR-based research. Infectious disease research See our growing catalog of sensitive, specific real-time PCR probe and primer sets for human viruses and other infectious disease research. Food pathogen detection Detect multiple bacteria in the same run, including Salmonella, Campylobacter, E. coli O157:H7, and Listeria monocytogenes. Waterborne pathogen detection Designed to detect and monitor waterborne pathogens in recreational and drinking water supplies. Pharmaceutical analytics Designed to detect mycoplasmas, viruses, and residual host cell contamination for pharmaceutical, cosmetics, and personal care product manufacturing. Stem cell research Solutions for analyzing stem cells, determining stemness, and studying gene regulation and translation in stem cells. Pharmacogenomics Predesigned TaqMan Assays for more than 175 ADME and CYP targets, including >95% of ADME core markers and a warfarin metabolism panel. Oncology and genetic disease research Enabling robust, reliable detection and quantitation of markers for cancer and genetic diseases. Plant sciences and agricultural biotechnology Instruments, reagents, and kits designed for plant researchers that enable remarkable agricultural discoveries—such as improved crops that feed more people to sustainable biofuels. Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 19 TaqMan chemistry and SYBR Green chemistry for real-time PCR TaqMan Assay reagents SYBR Green reagents Specificity High Low Sensitivity—low copy number High Variable* Reproducibility High Variable* Multiplexing Yes No Predesigned assays Yes No Custom assays Yes No User design and optimisation No Yes Cost High Low* Gene expression quantitation High Low DNA quantitation Yes Yes (pathogen detection) ChIP Yes Yes SNP genotyping Yes No MicroRNA Yes No Copy number Yes No Somatic mutation detection Yes No Pathway analysis Yes No Digital PCR Yes No *Depends on template quality, and primer design and optimisation TaqMan Assay—based detection SYBR Green—based detection Chemistry overview Uses a fluorogenic probe to enable detection of a specific PCR product as it accumulates during PCR cycles Uses SYBR™ Green I, or similar dye that binds to double-stranded DNA to detect PCR product as it accumulates during PCR We offer two types of chemistries to detect PCR products using real-time PCR instruments: • TaqMan Assay chemistry (also known as fluorogenic 5’ nuclease chemistry) • SYBR Green I dye chemistry Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 20 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Ordering information QuantStudio 6 Flex Real-Time PCR System configurations Cat. No. QuantStudio 6 Flex 96-well instrument, laptop configuration 4485689 QuantStudio 6 Flex 96-well Fast instrument, laptop configuration 4485699 QuantStudio 6 Flex 384-well instrument, laptop configuration 4485691 QuantStudio 6 Flex 96-well instrument, desktop configuration 4485692 QuantStudio 6 Flex 96-well Fast instrument, desktop configuration 4485697 QuantStudio 6 Flex 384-well instrument, desktop configuration 4485694 QuantStudio 6/7 Flex 96-well block upgrade kit 4453543 QuantStudio 6/7 Flex 96-well Fast block upgrade kit 4453544 QuantStudio 6/7 Flex 384-well block upgrade kit 4453545 QuantStudio 3 Real-Time PCR System configurations Cat. No. QuantStudio 3 96-well (0.2 mL block) instrument* A28137 QuantStudio 3 96-well Fast (0.1 mL block) instrument* A28136 QuantStudio 5 Real-Time PCR System configurations Cat. No. QuantStudio 5 96-well (0.2 mL block) instrument* A28139 QuantStudio 5 96-well Fast (0.1 mL block) instrument* A28138 QuantStudio 5 384-well instrument* A28140 QuantStudio 7 Flex Real-Time PCR System configurations Cat. No. QuantStudio 7 Flex 96-well instrument, laptop configuration 4485688 QuantStudio 7 Flex 96-well Fast instrument, laptop configuration 4485698 QuantStudio 7 Flex 384-well instrument, laptop configuration 4485695 QuantStudio 7 Flex TaqMan Array Card instrument, laptop configuration 4485700 QuantStudio 7 Flex 96-well instrument, desktop configuration 4485690 QuantStudio 7 Flex 96-well Fast instrument, desktop configuration 4485693 QuantStudio 7 Flex 384-well instrument, desktop configuration 4485701 QuantStudio 7 Flex TaqMan Array Card instrument, desktop configuration 4485696 QuantStudio 6/7 Flex 96-well block upgrade kit 4453543 QuantStudio 6/7 Flex 96-well Fast block upgrade kit 4453544 QuantStudio 6/7 Flex 384-well block upgrade kit 4453545 QuantStudio 7 Flex TaqMan Array Card block upgrade kit 4453546 * Does not include computer. Additional Cat. Nos. are available that include laptop or desktop computer. * Does not include computer. Additional Cat. Nos. are available that include laptop or desktop computer. 21 QuantStudio 12K Flex System configurations Cat. No. QuantStudio 12K Flex OpenArray block includes AccuFill™ System, laptop configuration 4471086 QuantStudio 12K Flex OpenArray block without AccuFill System, laptop configuration 4472379 QuantStudio 12K Flex 96-well instrument, laptop configuration 4471050 QuantStudio 12K Flex 96-well Fast instrument, laptop configuration 4471080 QuantStudio 12K Flex 384-well instrument, laptop configuration 4471081 QuantStudio 12K Flex, TaqMan Array Card instrument, laptop configuration 4471085 QuantStudio 12K Flex OpenArray block includes AccuFillSystem, desktop configuration 4471090 QuantStudio 12K Flex OpenArray block without AccuFill System, desktop configuration 4472380 QuantStudio 12K Flex 96-well instrument, desktop configuration 4471087 QuantStudio 12K Flex 96-well Fast instrument, desktop configuration 4471088 QuantStudio 12K Flex 384-well instrument, desktop configuration 4471134 QuantStudio 12K Flex TaqMan Array Card instrument, desktop configuration 4471089 QuantStudio 12K Flex 96-well block upgrade kit 4453543 QuantStudio 12K Flex 96-well Fast block upgrade kit 4453544 QuantStudio 12K Flex 384-well block upgrade kit 4453545 QuantStudio 12K Flex TaqMan Array Card block upgrade kit 4453546 QuantStudio 12K Flex OpenArray block upgrade kit with AccuFill System 4471067 QuantStudio 12K Flex AccuFill System 4471021 ProFlex™ Dual Flat PCR System 4484078 QuantStudio 3D Digital PCR System Cat. No. QuantStudio 3D Digital PCR System Package—includes: A25581 QuantStudio 3D Digital PCR instrument 4481097 QuantStudio 3D Digital PCR Chip Loader 4482592 QuantStudio 3D Digital PCR Chip Adapter Kit for Flat Block Thermal Cycler 4485513 QuantStudio 3D Digital PCR 20K Chip Pack (includes consumables) 4485507 ProFlex Dual Flat PCR System 4484078 QuantStudio 3D Digital PCR Master Mix (1.5 mL) 4482710 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems 22 Applied Biosystems | QuantStudio real-time PCR and digital PCR systems Support at every step Online Instrument Management Sign in to your thermofisher.com account to access the award-winning* free online Instrument Management** tool that enables faster responses to requests for service or service quotes, plus instant connection to key instrument and service information. Comprehensive instrument warranty Our factory-trained and certified field service engineers (FSEs) are focused on delivering the highest quality workmanship. During the warranty period all repairs, including engineer time and travel, are covered. Flexible service plans Choose from a variety of service options that balance your budget, productivity, uptime and regulatory requirements. Plans start with the most basic repair models and scale to premium offerings including advanced support and compliance services. On-site service plans are optimal for labs that have time-sensitive work and need to get their instrument back online quickly. These plans include guaranteed response times in most regions, scheduled planned maintenance and automatic software updates. The AB Repair Center plan is the cost-effective choice for customers who can allow their instrument to be sent away for repair—this plan provides a loaner instrument so that customers can maintain productivity while their instrument is being repaired. Compliance and validation services Our compliance and validation services are designed to help you balance business and regulatory requirements. From risk assessment and hardware/software qualification to full system validation, we partner with you to help mitigate regulatory risks and get your processes up and running. Training courses Our application and instrument training programs are led by scientists who aim to enhance your workday through experimental design best practices, workflow training and instrument troubleshooting. Hands-on classes are available at our Thermo Fisher Scientific training centers or in your lab. Technical support If you have questions about product selection or use, assay or experiment design, data analysis, or troubleshooting, contact our team of technical support scientists or access our online product and application support tools

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